Properties of shaker-type potassium channels in higher plants
This review summarizes the extensive data on the structure, function, membrane topogenesis, heteromerization, expression, localization, physiological roles and …

This review summarizes the extensive data on the structure, function, membrane topogenesis, heteromerization, expression, localization, physiological roles and …
The first voltage-gated potassium channels were cloned from Drosophila based on a mutation that causes the Shaker phenotype. They are composed of four transmembrane …
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Mortar or pill crusher; Glass shaker or cocktail shaker with a cover; Tea glass and straw; Ingredients. 6 lychees (peeled and seed removed) 3 lemon slices ... 0.4 g Monounsaturated Fat: 0.1 g Sodium: 16 mg Potassium: 660 mg Fiber: 10 g Sugar: 17 g Vitamin A: 71 IU Vitamin C: 215 mg Calcium: 87 mg Iron: 2 mg. Tried this recipe? Let us …
To understand the molecular mechanisms responsible for generating physiologically diverse potassium channels in mammalian cells, mouse genomic clones have been isolated with a potassium channel complementary DNA, MBK1, that is homologous to the Drosophila potassium channel gene, Shaker.A family of three …
Cellular localization and comparison of the functional, biophysical and topological characteristics of the plant Shaker-type K + channels demonstrate that in …
Given that Shaker is likely a gene that encodes a potassium channel, we noted in the last paragraph of our 1977 paper 3: "… this may open the possibility of isolating the molecular entity ...
We have determined the crystal structure of the T1 domain of a Shaker potassium channel at 1.55 Å resolution. The structure reveals that four identical subunits are arranged in a four-fold ...
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These studies, including the finding that treating wild-type animals with the potassium channel blocker 4-aminopyridine (4-AP) could phenocopy the Shaker mutant phenotypes, led us to propose...
A region near the amino terminus with an important role in inactivation has been identified and the results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation. The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the …
In this method, proteoliposomes containing Shaker potassium channels are synthesized in vitro and injected into the oocytes, yielding functional preparations as shown by electrophysiological and ...
Crushed red pepper is not a great source of any minerals but it contains several in low concentrations. You can get minerals like iron, manganese, and potassium from a serving of crushed red pepper. Fiber . You can use crushed red pepper to get more fiber in your diet. A 1-tablespoon serving can provide you with more than a gram of …
K+ channels of the Shaker family have been shown to play crucial roles in K+ uptake and transport. Cotton (Gossypium hirsutum) is an important cash crop. In this study, the 24 Shaker family genes were identified in cotton. Phylogenetic analysis suggests that they were assigned to five clusters. Additionally, their chromosomal location, …
Voltage-activated potassium (Kv) channels open and close in response to changes in membrane voltage, serving critical functions in electrical signaling in neurons and muscle, neurotransmitter and hormone secretion, cell proliferation and migration, and ion homeostasis (1, 2).Functional studies of Shaker Kv channels have been …
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The conformational changes associated with activation gating in Shaker potassium channels are functionally characterized in patch-clamp recordings made from Xenopus laevis oocytes expressing Shaker channels with fast inactivation removed. Estimates of the forward and backward rates for transitions are obtained by fitting …
This tetrameric organization closely resembles the structure of the Shaker potassium channel, which is composed of four tandemly associated homologous domains. 103,104 The clustering of the 4 subunits is thought to create an aqueous pore centered at the fourfold symmetry axis. 104,105 This proposed tetrameric architecture implies that …
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Shaker potassium channel complexes are thought to be composed of 4 alpha and 4 beta subunits. Cloning and Expression By PCR of a human hippocampal library with degenerate primers based on conserved regions of rat beta-1 (KCNA1B; 601141) and beta-2 subunits, McCormack et al. (1995) isolated cDNAs encoding human beta-1 and …
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In this paper, we extend the kinetic analysis of the Shaker channel to the effects of the V2 mutation. This characterization of V2 serves two purposes. First, it …
15 August 2022. 35 years of channelling potassium ions. In 1987, two independent groups cloned a gene that encoded the potassium-ion-channel protein Shaker, enabling functional and...
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A Shaker Potassium Channel is a type of potassium channel that has been identified as a fundamental regulator of sleep behavior in organisms like fruit flies and mammals. In humans, auto-immunity to Shaker-type potassium channels can lead to conditions such as Morvan's syndrome, which may include symptoms like insomnia. ...
Gating of single Shaker potassium channels in Drosophila muscle and in Xenopus oocytes injected with Shaker mRNA. Proc Natl Acad Sci U S A. 1989 Sep; 86 (18):7243–7247. [PMC free article] [Google Scholar] Zagotta WN, Hoshi T, Aldrich RW. Restoration of inactivation in mutants of Shaker potassium channels by a peptide …
Potassium should not be given in dextrose-containing solutions because dextrose-stimulated insulin secretion can exacerbate hypokalemia. Nonurgent hypokalemia is treated with 40 to 100 mmol
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